The IC50 curves were calculated using variable slope for log (inhibitor) vs. AM (~0. siRNA-mediated knockdown of KLF2 in RAW 264. ® reader to the computer. Note: In this user guide, all references to SpectraMax Multi-Mode. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of feature variability. When a feature or capability applies to only certain readers, this exception is noted. Operated from(A) ROS levels and (B) NAD+/NADH ratios in rotenone-pretreated young HDFs. . The M4 Reader offers the same modes as the M3 Reader, plus time-resolved fluorescence, and can be upgraded to include fluorescence polarization. The conjugate was also characterized using FTIR (FT/IR-4200, Jasco Inc. However, dynamic changes in soil microbiota under different tree ages have received little attention. Raw data generated on SpectraMax M4 Multi-Mode Microplate Reader is shown in the table. Plus 384 ABS1 Location: Reader Validation-Plate Abs A reminder about recertification was added. Page: 1/6. Lysosomal proteolytic activity. Learn how to easily set up an efficient and informative plate read by using SoftMax Pro 6 Microplate Data Acquisition and Analysis software. Supplier: Molecular Devices. ). We therefore gathered salient. ATP concentration (M) Average RLU SpectraMax L SpectraMax i3x (cartridge) SpectraMax Mini SpectraMax iD5 SpectraMax i3x (onboard) SpectraMax M5eThe SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. 95. To report an issue with this product, Brief content visible, double tap to read full content. 2. Choose from a three- (M3), four- (M4), orThe optical density was determined spectrophotometrically at 405 nm using a SpectraMax M4 microplate reader (Molecular Devices, Sunnyvale, CA). 305 reference level saturation. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of feature variability. 306 plate air cal fail,low light. S. Full content visible, double tap to read brief content. Italic and boldface type are used for emphasis. Choose from a three- (M3), four- (M4), or Cables. 39. Images were taken using a fluorescence microscope (Nikon 80i, Japan). Products : SpectraMax M2/M2e reader, SpectraMax M4 reader, SpectraMax M5/M5e reader, Gemini XPS reader, SpectraMax 190 reader, VersaMax reader, Gemini EM reader, SpectraMax Plus 384 reader. 116 SpectraMax M5 and M5e Multi-Mode. The combination index (CI) was calculated. The SpectraMax M4 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96 and 384. The content was vortexed for 1 min and incubated in the dark for 20 min at 25°C. 75 × 10 5) suspended in PBS in 96-well dark plates incubated with NP or SiO 2 for 1 h at 37 °C. Roelli1, Amandine Stoss1, Rebecca A. The SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. 05 mg mL −1. 7 cells. The innovative and flexible design features. In the competitive field of drug development where timely go/no-go decisions must be made to bring effective class-leading drugs to market, it is vital to work with a CRO that can provide highly. Cell viability was calculated using the following formulaThe luciferase signal was read in a plate reader (SpectraMax M4; Molecular Devices, Sunnyvale, CA). FlexStation™ III. The optical absorbance of the plate at 450 nm was measured using a multifunctional microplate reader SpectraMax M4 (Molecular Devices, LLC, San Jose, CA, USA). alamarBlue reagent volume in a different spheroid microplate formatA SpectraMax M4 microplate reader (Molecular Devices, LLC, Sunnyvale, CA, USA) was used to measure the absorbance at a wavelength of 450 nm. GSA System Includes: SpectraMax M4 Multi-Mode Spectrophotometer with Tunable. 6: bioNova científica, s. Cellular viability was assessed by using resazurin at 1. Plot the fluorescence intensity versus the cell seeding number. SpectraMax Gemini XPS/EM Register or Sign In. 4. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. 2. TEM. For the SpectraMax M3/M4/M5 readers - use the Purple Assay Plate adapter for Top Read only. Finally, the optical density was evaluated using SpectraMax M4 plate reader (Molecular Devices, San Jose, CA, USA) at 450 nm. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quantitation of firefly luciferase expression in mammalian cells. The fluorescent signal was measured by multiplate reader (Spectramax M4, Molecular Devices LLC, Sunnyvale, California, USA). Analytical Technologies Group has deep experience maintaining and repairing Molecular Devices microplate readers, including the SpectraMax® M Series microplate readers (M2, M2e, M3, M5, M5, and M5e). Table 2. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v2. The SpectraMax Quant dsDNA Assay Kits provide pre-optimized, complete solutions that are sensitive, accurate, and easy to use. 2 only). A microplate reader that can detect two or more applications is considered a multi-mode plate reader. . The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Z’-LYTE® Assay using the Z'-LYTE® Tyr6 kit. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a later time. book Page 1 Wednesday, July 7, 2010 2:09 PM SpectraMaxM3,M4,M5,andM5eMicroplateReaderUserGuide 8 0112-0115G CAUTION!Useoforganicsolventscancauseharmtotheopticsintheinstrument. Then, MTT (10 μl of 5 mg/ml) was added to each well and further incubated for 4 h. Raw data generated on SpectraMax M4 Multi-Mode Microplate Reader is shown in the table. alamarBlue reagent volume in a different spheroid microplate formatAfter incubating for 10 min at room temperature, the absorbances of standards and samples were measured at 450 and 590 nm, 12 using a Spectramax M4 spectrophotometer (Molecular Devices). For problems with SpectraMax Multi-Mode Microplate Readers that are not listed here, in the U. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a later time. Size and zeta potential of nanoparticles were measured by Zetasizer Nano ZS90 (Malvern Instrument). SpectraMax® ABS Plus. In. Raw data generated on SpectraMax M4 Multi-Mode Microplate Reader is shown in the table. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). The SpectraMax Quant dsDNA Assay Kits provide pre-optimized, complete solutions that are sensitive, accurate, and easy to use. Turn the instrument around so that the back of the instrument is. Microplate Reader Shelf. This assay kit is optimized for the SpectraMax i3x Multimode Microplate Reader with a preconfigured protocol in SoftMax Pro Software for rapid. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. 5. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. , USA). The test conditions are as follows: mobile phase: acetonitrile/H2O (0. Hemoglobin release from erythrocyte lysis was detected by measuring the optical density (OD) of the supernatant at 412 nm using a microplate reader (SpectraMax M4 from Molecular Devices, or Tecan SPARK, from Tecan, Männedorf,. The chamber contains springs that position the cuvette in the proper alignment for a read. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual- monochromator microplate reader platform offering a wide range of. SpectraMax Paradigm SpectraMax i3 SpectraMax Flex-Station 3 SpectraMax F5 SpectraMax M4, M5, and M5e SpectraMax M2, and M2e: Secondary modes: BMG LABTECH: CLARIOstar PHERAstar FS PHERAstar Plus FLUOstar Omega POLARstar Omega PerkinElmer: EnSpire® Multimode Plate Reader EnVision Multilabel Plate. With only a few product lines that we provide service for, our engineers have unrivaled expertise in the SpectraMax® microplate readers. FilterMax F3 and F5 Multi-Mode; SpectraMax Paradigm® Multi-Mode. For more detailed information and technical support of Molecular Devices instruments or software, Luciferase Detection Instrumentation Guide. The absorbance of formazan was detected at 540-nm wavelength with a reference wavelength at 650 nm for background using a SpectraMax M4 plate reader (Molecular Devices). Table of Contents. Background-subtracted data values (mean +/- SD) are graphed. 2. book Page 1 Wednesday, July 7, 2010 2:09 PM M4 SpectraMax M5 SpectraMax M5e Absorbance FluorescenceIntensity Luminescence Time-Resolved Fluorescence FluorescencePolarization ReadModes The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. All associated StakMax® stackers. 2. The ELISA kits (Lianke Bio, China) were used to detect the concentration of specific cytokines. Italic and boldface type are used for emphasis. Calpain activity assay The cells were treated with different drugs for 24 hours and were resuspended in 100 μL extraction buffer and homogenized by pipetting. Subsequently, 20 µl CCK-8 solution (5 mg/ml) diluted in PBS was added to each well for another 1 h and the absorbance was measured at 450 nm using a SpectraMax M4 microplate reader (Molecular Devices, LLC). High tolerance for contaminants. Preconfigured SoftMax® Pro Software protocols (available at softmaxpro. 0397 in x 3. F1 SpectraMax Plus384andABSPlus: Certificate Value – 2≤ Average Peak Value≤ Certificate Value + 2 SpectraMax M2,M2e,M3,M4,M5,andM5e: Certificate Value – 3≤ Average Peak Value≤ Certificate Value + 3 Photometric Accuracy F2, F3, F4 AverageOD=Certificate Value± (Certificate Value(0. The microplate was exposed to an excitation wavelength of 530 nm (peak excitation is 540 nm), and the emission at 590 nm (peak emission is 580 nm) is recorded. 3 or higher) SpectraMax® M5 and M5e Multi-Mode Microplate Readers; SpectraMax® M4 Multi-Mode Microplate Reader; SpectraMax® M3 Multi-Mode Microplate Reader; SpectraMax® M2 and M2e Multi-Mode Microplate Readers; SpectraMax® 340PC 384 Absorbance Microplate Reader The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. Dead Time Correction accounts for the finite amount of time it takes the instrument to detect and then record the absorbance and counting of a photon by the. The microplate was exposed to an excitation wavelength of 530 nm (peak excitation is 540 nm), and the emission at 590 nm (peak emission is 580 nm) is recorded. Serial number location on Series II and Multi-Mode plate readers. Results. SpectraMax® Paradigm® Multi-Mode Detection Platform. In vitro transcription (IVT) is an essential technique for RNA synthesis. MTT values at day 3 were normalized to day 0. . Residual. One of the. The MTT reagent was incubated with cells for 4 h. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. Luminescence was immediately detected by the SpectraMax M4 Microplate/Cuvette Reader (Molecular Devices, San Jose, CA, USA). org) with optimized instrument settings (Table 1) were used to generate and analyze data. FD-4 concentrations in plasma were analyzed in duplicates using a spectrophotometer (SpectraMax M4, Molecular Devices, San Jose, CA, USA) with excitation λ 485 nm and emission λ 535 nm. 116 SpectraMax M5 and M5e Multi-Mode. org) with optimized instrument settings (Table 1) were used to generate and analyze data. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. The polysaccharide HPA with different concentrations (0, 25, 50, and 100 μg/mL) was added and incubated with cells for 24 h. Dettmer4, Wayne A. 4) and cells were stained with 150 nM TSQ for 1 min. Plates were then read at 450 nm using a SpectraMax M4 device (Molecular Devices, Sunnyvale, CA). Examples: “Press carefully to engage,” “ Do not press down. These settings are also valid for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. Find M4 Group Marketing Specialists in Victoria, with phone, website, address, opening hours and contact info. 316 grating motor driver faulty. The OD value of each well was determined using a SpectraMax M4 Multimode Microplate reader (Molecular Devices, USA), and the concentration of cytokines was calculated by comparing their OD value with the corresponding standard curve. Danaher Inc spectramax m series Spectramax M Series, supplied by Danaher Inc, used in various techniques. The enzymatic reaction was triggered by addition of luminol and the chemiluminescence signal was monitored at 37 °C using a microplate reader SpectraMax M4 (Molecular Devices, Sunnyvale, CA, USA), with one acquisition every 2 s for 300 min. The absorbance change at 620 nm was measured by a SpectraMax M2 Microplate Reader (Molecular Devices). The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. 4. In the M2, M5 and Plus 384, the light. The 96-well plates were read on the SpectraMax M4 plate reader at the following wavelengths: green fluorescence at 488 ex. RFU was plotted versus time to derive the rate of formation of the fluorophore. Where is the instrument serial number located? The serial number is on. 01) + 0. In this application note, we demonstrate the measurement of luciferase expression in CHO-K1 cells using the SpectraMax Glo Steady-Luc Reporter Assay Kit, which affords long-lasting luminescence signals. Following incubation, the media was aspirated completely and MTT formazan crystals formed were dissolved by the addition of dimethylsulfoxide, and the reading was taken at 570 nm using SpectraMax M4. To assess the effect of CAD drugs on lysosomal activity in BMdM, fluorescence analysis using the DQ Green BSA probe was performed. SpectraMax Microplate Readers. For more detailed information and technical support of Molecular Devices instruments or software,Luciferase Detection Instrumentation Guide. The ELISA kits (Lianke Bio, China) were used to detect the concentration of specific cytokines. Finally, we have two New Brunswick Excella E24 shaking incubators, one with refrigeration capabilities and a Thermo Sciences -80˚C freezer. The aim of the present study was to investigate the preventive effects of. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. The use of gene reporters such as luciferase permits highly sensitive and nondestructive monitoring of gene expression. After incubation, the biofilms were washed with PBS until fully remove crystal violet solutions and then, solubilized by adding a 150 μL acetic acid. qPCR The jejunum was sampled immediately after termination, snap frozen, andChoose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a later time. ” The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Next to the pushbutton is a green LED. Steady-state kinetics measurements were performed at 25°C in 96-well plates (Corning) using a SpectraMax M4 spectrophotometer (Molecular Devices) in PBS buffer. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. 2 pM 2 pM 384-well (50 µL) 3 pM 4 pMCell viability was determined by addition of MTT ([3-(4,5)-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide, Sigma) to cells and incubation for 4 h. 5. Serum C-terminal telopeptide of type I collagen (S-CTX), osteocalcin (BGP), and tartrate-resistant acid phosphatase (TRAP) levels in serum and the receptor activator of the NF-κB ligand (RANKL), osteoprotegerin (OPG), and RANK levels in marrow were determinedThe ELISA kits (Lianke Bio, China) were used to detect the concentration of specific cytokines. Prepare 22 mg/mL of FD4, 16 mg/mL of rhodamine70, and 40 mg/mL of creatinine in sterile PBS. On day 2, in the morning weight the mice. WARNING! The instrument weighs approximately 36 pounds (16. Colony formation assays were performed to measure cell proliferation. 0. *Statistical significance: p < 0. Statistical analysis. , San Jose, CA) was. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. SpectraMax ABS Plus SpectraTest ABS2 v1. Products : SpectraMax M4 reader, FilterMax F5 reader, SpectraMax Paradigm reader, SpectraMax M5/M5e reader Do you know any applications for TRF/HTRF that differ from Fluorescence Polarization? TR-FRET assays are well-suited for protein:protein interactions. SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. 2. 6 reviews. The SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading 312 gain calibration failed. The SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. Like all. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader. ISM Equipment Classification (Group 1, Class A) This equipment is. 7. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. ROS estimation by 2′,7′- dichlorofluorescin diacetate (DCFDA) staining . Then 10 µL of CCK-8 reagent was added to each well, and cell growth was measured at 490 nm using the SpectraMax M4 Multimode Microplate reader (Molecular Devices, San Jose, CA, USA). Ltd. , 2011). These samples were analyzed using a multimode plate reader (SpectraMax M4, Molecular Devices, USA). All configurations. Cell viability was calculated using the formula as follows: (OD450 KO HepG2 -OD450 blank /OD450 WT HepG2 -OD450 blank ) × 100%. When using a SpectraMax M3 Reader, the GxP audit trail incorrectly reported the instrument as a SpectraMax M4 Read er. After incubating for 10 min at room temperature, the absorbances of standards and samples were measured at 450 and 590 nm, 12 using a Spectramax M4 spectrophotometer (Molecular Devices). Each well was scanned at nine evenly distributed points and averaged automatically with the software. Table 2. Table 2. Microplate Readers include the M3, M4,. Establishment of mouse orthotopic xenograft bladder tumor model. The absorption value at 570 nm was measured using a Molecular Devices SpectraMax M4 plate reader, and the absorbance at 690 nm was subtracted as the background. Formazan crystals formed were solubilized in 10 μl DMSO, and absorbance was recorded using a multimode plate reader (SpectraMax M4; Molecular Devices, USA) at 606-nm wavelength. Allows you to use EOS lenses on a micro four thirds camera M4/3 K&F concept lens adapter. Members of the Sphingomonadales are renowned for their ability to degrade polycyclic aromatic hydrocarbons (PAHs). , UK) or SpectraMax M4 by Molecular Device. Bioz Stars score: 98/100, based on 1 PubMed citations. Molecular Devices SpectraMax M4 upgradable microplate/cuvette reader (90V to 240V) with current version of SoftMax Pro. Qualify the luminescence performance of the SpectraMax . Large Molecule Bioanalysis. Resolution: In the GxP audit trail, the SpectraMax M3 and M4 Readers are now identified correctly. normalized response (Graphpad Prism 8. Measurements of temperature increase (ΔT) during the exposure of MNP/AMF hyperthermiaThe culture medium was removed, and the blue product was dissolved in DMSO (100 μl). N2a cells were induced for neurite growth by adding nerve growth factor (NGF) (50 ng/ml). com. FD-4 concentrations in plasma were analyzed in duplicates using a spectrophotometer (SpectraMax M4, Molecular Devices, San Jose, CA, USA) with excitation λ 485 nm and emission λ 535 nm. Phillips5, Martin A. Also, when using a SpectraMax M4 Reader, the GxP audit trail incorrectly re ported the instrument as a SpectraMax M3 Reader. Lastly, the TMB substrate (Beyotime) was added, and absorbance was measured at 450 nm by a microplate reader (SpectraMax M4). 1. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Environmental Chamber and Respiratory SimulatorsRhizoma drynariae, a traditional Chinese herb, is commonly used in treatment of bone healing in osteoporotic fractures. The absorbance was measured spectrophotometrically using a microplate reader (SpectraMax M4, Molecular Devices, CA, USA) at 570 nm. Note: In this user guide, SpectraMax ® refers to several SpectraMax . 383 inches whereas the maximum allowed is 5. 11. spr * SpectraMax i3(x) SpectraTest ABS2 v1. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. . Products : SpectraMax M4 reader, FilterMax F5 reader, SpectraMax Paradigm reader, SpectraMax M5/M5e reader Do you know any applications for TRF/HTRF that differ from Fluorescence Polarization? TR-FRET assays are well-suited for protein:protein interactions. 2. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. Spectramax M Series, supplied by Danaher Inc, used in various techniques. • SpectraMax® M3/M4/M5/M5e Multi-Mode Microplate Readers • SpectraMax® Paradigm® Multi-Mode Microplate ReaderThe medium was replaced with CM from the macrophages of the different groups and then incubated for 48 or 72 h. FP assay do not perform well with protein:protein interactions. 2. Living cells were then incubated with 100 µl of medium assay provided by the assay kit. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. The activity of untreated cells was normalized to 100% and the treated cell activity was compared with that. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. SpectraMax M3 Reader SpectraMax M4 Reader SpectraMax M5 Reader SpectraMax M5e Reader TRF,HTRF,FP HTRF, FP HTRF N/A Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well. Samples (2 mg) were mixed with potassium bromide in a 1:100 ratio before the analysis. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Molecular Devices recommends that you use high-quality, doubleshielded cables to connect your SpectraMax. Background-subtracted data values (mean +/- SD) are graphed. Preconfigured SoftMax ® Pro Software protocols (available at softmaxpro. TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode. 73. Ninety microliters of 2-chloro-4-nitrophenyl-α- d -maltotrioside substrate dilutions from 5000 to 1. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port. alamarBlue reagent volume in a different spheroid microplate formatA SpectraMax M4 microplate reader (Molecular Devices, LLC, Sunnyvale, CA, USA) was used to measure the absorbance at a wavelength of 450 nm. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. Description 1. com. 光验证板,用于验证 SpectraMax M3、M4 和 M5/M5e 多功能读板机的光 学性能表现。 SoftMax Pro 内置 140 多种预存实验方法可以直接调用,可以进行数 据获取和个性化的数据分析;强大的图标绘制工具和文本编辑功 能,可以方便的进行各种数据结果的展示。 完整的. Example: [Drawer]. 1. Choose from a three- (M3), four- (M4), orThe integration time suggested is 5-10 sec. We therefore gathered salient. Published Dec 16, 2020 | Updated Jan 25, 2021 Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, Gemini XPS reader, SpectraMax M5/M5e reader, SpectraMax. . 50 μl of samples were added into the mixture of 40 μl of detection buffer and 10 μl of Ac-DEVD-pNA (2 mM) with gentle shaking for 5 min. free. Background-subtracted data values (mean +/- SD) are graphed. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and. 2. Example: [Drawer]. The combination index (CI) was calculated. Free essays, homework help, flashcards, research papers, book reports, term papers, history, science, politicsSpectraMax Spectrofluorometers. Fifteen different automated tests are provided: • Background noise • Background spike • Lower limit of detection • Crosstalk • Linearity • Relative luminescence units (RLU) • Kinetic noise (low signal)0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideSpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. . The fluorescence intensity was measured as ratio of values at an excitation at 340 nm and 380 nm with an emission at 510 nm, using Spectramax M4 (Molecular Devices). . Lower limits of detection for ATP determined using the ATPlite . Optimized for fluorescence microplate readers. The following document is intended to demonstrate setup of this instrument. Also, when using a SpectraMax M4 Reader, the GxP audit trail incorrectly re ported the instrument as a SpectraMax M3 Reader. For problems with SpectraMax Multi-Mode Microplate Readers that are not listed here, in the U. Large molecule bioanalysis, immunogenicity and PK testing with higher quality data, rapid turnaround and fewer surprises. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading capability. Vol 4, Iss 22, Nov 20, 2014 . Cet appareil ISM est confomre à la norme NMB-001 du Canada. . The lower critical solution temperature (LCST) at which phase transition occurs was measured via plate reader absorbance for n = 3 samples (SpectraMax M4; Molecular Devices). 313 reference gain check fail. The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuide SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Setting up the Instrument 1. Hexokinase reagent (Eagle Diagnostics) was added and the reaction absorbance was read at 340 nm using a SpectraMax M4 multimode plate reader (Molecular Devices). When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. However, little is known about the regulatory mechanisms of the degradative pathway. Note: In this user guide, SpectraMax ® refers to several SpectraMax . Laboratory Freezers. SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. At sacrifice, serum was isolated from mouse total blood and maintained at –80 °C. The fluorescent signal at an excitation wavelength of 530 nm and emission wavelength of 580 nm was measured by Spectramax M4, USA. At 48 h after transfection, the Nano-Glo Live Cell Assay reagent was added and the luciferase activity in the medium and cells were measured using a SpectraMax M4 (Molecular Devices, San Jose, CA, USA). Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening. SPECTROMAXx with iCAL 2. The M3/M4/M5/M5e/Flex3 and the L/LMax II/LMax II 384 groups of readers each require different Dead Time Correction values, and therefore each has their own Certification. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. For more detailed information and technical support of Life Technologies assays, please call 1-800-955-6288 and enter extension 40266 or email [email protected]. Technical specifications Detection limit for ATP Glow 0200-7012 (LUM) Optimized Guaranteed* 96-well (200 µL) 1. Bioz Stars score: 86/100, based on 1 PubMed citations. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, SpectraMax M4 reader, SoftMax Pro Software, SpectraMax Plus 384 reader, SpectraMax 190 reader, SpectraMax 340PC 384 (Obsolete), SpectraMax M5/M5e reader. 5 mg mL −1 with 0. SpectraMax® Paradigm® Multi-Mode Detection Platform. D 1 1. 37. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of feature. Add To My List; Create New List; Quickview. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader. MEK Inhibition Induces Therapeutic Iodine Uptake in a Murine Model of Anaplastic Thyroid Cancer Oussama ElMokh1, Vincent Taelman2, Piotr Radojewski2, Matthias A. 0 statistics software. Real time thermal cyclers (iCycleriQ apparatus with iCyclerOptical System software version 3. Images were taken using a fluorescence microscope (Nikon 80i, Japan). The following document is intended to demonstrate setup of this instrument. MTT was then removed, and the formed formazan crystals were then dissolved in 150‐μl DMSO. Parameter SpectraMax iD5 SpectraMax i3x SpectraMax. C. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. Using cross-feeding bioassay, a functional LuxI/LuxR-type acyl-homoserine lactone (AHL)-mediated quorum sensing (QS) system. SpectraMax® M4 Multi-Mode Microplate Reader MLDVM4-Dual monochromatic optics -Wide range of applications -Top or bottom read modes The SpectraMax M3 Multi-Mode Microplate Reader provides three modes of detection in one platform The SpectraMax M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range. . Dagda Lab. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. There are multiple vendors. Multi-Mode Microplate Readers. TEM. SpectraMax M2 and M2e Performance Specifications SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. Dumont3, Matthias S. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. GSA System Includes: SpectraMax M4 Multi-Mode Spectrophotometer with Tunable. The adherent cells were incubated in 100 μl of lysis buffer on ice for 20 min. MIC assay: After diluting colloidal nanoparticle solutions of varying concentrations in TSB, they were injected onto a 96-well plate with a strain of bacteria at a concentration of 10 6 CFU/mL. If you need to relocate a SpectraMax Multi-Mode Microplate Reader, follow these steps. Products : SpectraMax M3 reader, SpectraMax M4 reader, SpectraTest Validation Plates LM1 , FlexStation 3. Serum C-reactive protein (CRP) was quantified using the Mouse C-Reactive Protein/CRP Quantikine ELISA Kit. , USA). 2. > spectramax m4 microplate reader. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, SpectraMax M4 reader, SpectraMax M3 reader MTT reduction - a tetrazolium-based colorimetric assay for cell survival and proliferation (Maxline Application Note #5) (PDF) Summary: The MTT assay measures cell respiration and the amount of formazan. 3, La Jolla, CA, USA). book Page 1 Wednesday, July 7, 2010 2:09 PMLuciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. The optical density was measured using a microplate reader (Spectramax M4, Molecular Devices, San Jose, CA, USA) at 570 nm. Description 1. The medium was then removed and 100 μL of DMSO was added to dissolve the formazan crystal. Table 1. Exhibit with us {{>currencyLabel}} Back. The SpectraMax M4 reader had identical performance specifications to the SpectraMax M5 reader for absorbance and fluorescence detection. All experiments. We have two NanoDrop micro-scale spectrophotometers, a Molecular Devices Spectramax M4 micro plate reader and a UVP gel documentation camera. Cytosolic [K +] was then monitored using a SpectraMax M4 spectrofluorometer (San Jose, CA, USA) by loading AM at 37 ℃ for 40 min with 12 µM APG-2. data generated on SpectraMax M4 Multi-Mode Microplate Reader is shown in the table. SpectraMax M2 and M2e Performance Specifications SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. reader (SpectraMax M4, Molecular Devices; Sunnyvale, CA, USA) at 540 nm absorbance. Methods for the accurate and rapid screening of IVT conditions will facilitate RNA polymerase engineering, promoter optimization, and screening for new transcription inhibitor drugs. The reaction systems were cultured in 37°C for 1 h, and the OD405 values were read on SpectraMax M4 (Molecular devices). The SpectraMax M4 reader had identical performance specifications to the SpectraMax M5 reader for absorbance and fluorescence detection. Transfer plate to SpectraMax M5 with excitation monochromator set to 455 nm with 10 nm bandwidth, emission monochromator set to 525 nm with 10 nm bandwidth, and 515 nm cutoff filter. SpectraMax M3 Reader SpectraMax M4 Reader SpectraMax M5 Reader SpectraMax M5e Reader 5 B > 4 4 5 B 5 : F 8 8 > 3 ; > I 5 = 8 5. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. 3 or higher) SpectraMax® M5 and M5e Multi-Mode Microplate Readers; SpectraMax® M4 Multi-Mode Microplate Reader; SpectraMax® M3 Multi-Mode Microplate Reader; SpectraMax® M2 and M2e Multi-Mode Microplate Readers; SpectraMax® 340PC 384 Absorbance Microplate ReaderThe SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. 72 MB. In. SpectraMax® Paradigm® Multi-Mode Detection Platform. org) with optimized instrument settings (Table 1) were used to generate and analyze data. 0112-0174C 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 InstrumentFeatures 8 ComputerIntegration 9 PMTTechnology 9SpectraMax® i3xMulti-ModeMicroplateReader SpectraMax® i3Multi-ModeMicroplateReader FlexStation® 3Multi-ModeMicroplateReader SpectraMax® M5eMulti-ModeMicroplateReader SpectraMax®M5Multi-ModeMicroplateReader SpectraMax® M4Multi-ModeMicroplateReader SpectraMax® M3Multi. The data represent the mean ± SD of three independent experiments. To determine the cell viability, thiazolyl blue tetrazolium bromide (MTT) assay was performed. Here, we present an approach to predict UV-visible absorption spectra of solvated aromatic molecules by quantum chemistry (QC) and machine learning (ML). Molecular Devices SpectraMax M4 upgradable microplate/cuvette reader (90V to 240V) with current version of SoftMax Pro. STAKMAX. SpectraMax® M4. SpectraMax M3 Multi-Mode Microplate Reader with 1-Year Service Contract. TSQ was dissolved in Lock’s buffer (pH 7.